Thioredoxin

Abstract:
The sequence of human thio oxidoreductionin(TRX) contains 2 pairs of cysteine residues, one at positions 32 and 35, the other at positions 69 and 73. This experiment was performed to model which cysteine residues is responsible for redox activity. The sequence of thioredoxin was altered by the PCR reaction by changing two pairs of cysteine codons to two serine codons. The mutated deoxyribonucleic acid fragments which were 1SS and 2SS were free and cloned into a plasmid vector. The clones inserted with mutated deoxyribonucleic acid fragments were selected by a saturation reaction. The required size of insertion was confirmed by the breastwork digestion. SDS-PAGE was used to confirm the induction while western malignment was used to identify the induction of thioredoxin protein. The purity of different forms of thioredoxin proteins( haywire type, 1SS, 2SS) were determined by SDS-PAGE after purified by ion exchange chromatography. The plug spectroscopy was used to determine the molecular weight of thioredoxin proteins and the redox activity of different forms of thioredoxin proteins were measured by insulin reduction assay. Compared to ill-advised type and 2SS, 1SS shows no redox activity based on the reading of OD value in insulin reduction assay. The quick site was determined to be the first pair of cysteine residues.





basis:
Thioredoxin is a cytosolic protein which functions as a protein disulphide reductase. The size is about 12kDa. It is waken stable and first isolated from Escherichia coli(Laurent et al 1964). Its reduced enunciate is kept by thioredoxin reductase which is NADPH-dependent(Laurent et al 1964). Thioredoxin has different intracellular and extracellular functions such as cell growth, apopotosis, immune response and arrangement regulation. The activity of thioredoxin depends on a active site derived from two cysteine residues. One is at amino acid positions 32 and 35 and the other is at position 69 and 73.
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Figure(1) Redox reaction of...If you want to get a full essay, order it on our website: Orderessay



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